FastQCFastQC Report
Wed 3 Jul 2024
SRR6820491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6820491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14099347
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC740590.5252654608755994Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCTCTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTGTCCAT696070.4936895304442113No Hit
CTGGAATATCAACCAGTTGTCCATCGCCTACGCATTCTCTGCCTCGGCTT580200.4115084195033997No Hit
CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTT534230.3789040726496057No Hit
CCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATC450330.31939777069108233No Hit
CTTCGAAACAACCGGCGGATTTCCCTACCGGTCTATCTACCTCCTTTGGA422430.2996096202185818No Hit
CTTTACTTGAGGCTAGCCCTAAAGCTATTTCGGGGAGAACCAGCTATATC377660.2678563766109168No Hit
AATGCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAGCGGT334710.2373939729265476No Hit
CTTCAGGTGGCACTGGAATATCAACCAGTTGTCCATCGCCTACGCATTCT334390.23716701206091317No Hit
GTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGT323380.22935813977767905No Hit
CTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATAC304490.21596035617819748No Hit
CGAACCTTCCCCAGGAAACCTTAGGCTTTCGACGGTAAAGATTCTCACTT295970.20991752313068113No Hit
CTTCGCTCAAACGGCTTCAGGTGGCACTGGAATATCAACCAGTTGTCCAT291250.20656985036257353No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT287550.2039456153536756Illumina Single End PCR Primer 1 (97% over 34bp)
CTACTGTCTCGTCCACATGTCCTTCCGGTCATGCTTCGGCCTACCACAGT286960.20352715625766216No Hit
CACTTCTCTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTGTCC277530.19683890324849795No Hit
CTTATGAGTACTTAACCTCGCCGCATACAATAACTCGCCGGACCGTTCTA275760.1955835259604576No Hit
CGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGG272570.19332100983116451No Hit
CCCCACTTCTCTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTG248500.17624929721922583No Hit
GTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACT243950.17302219741098648No Hit
CCCACTTCTCTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTGT236630.16783046760959922No Hit
ATGCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAGCGGTA232950.16522041765480344No Hit
CCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG224670.15934780525651296No Hit
CACGGCAGTATGCTGACCTGCCATTACTAGCAATTCCGACTTCATGCAGG219460.1556525986629026No Hit
ATTCACGGCAGTATGCTGACCTGCCATTACTAGCAATTCCGACTTCATGC217010.15391492953538913No Hit
CTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTGTCCATCACCT207900.14745363739185935No Hit
GTAATGCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAGCG197160.13983626333900429No Hit
TGCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAGCGGTAA195610.13873692164608759No Hit
CAGGAATTTCAACCTGTTGTCCATCACCTACGCCTTCCGGCCTCGGCTTA188810.13391400325135625No Hit
CTTATTCCGGCATTCTCACTACTGTCTCGTCCACATGTCCTTCCGGTCAT188170.13346008152008743No Hit
CTCACTACTGTCTCGTCCACATGTCCTTCCGGTCATGCTTCGGCCTACCA182870.12970104218301742No Hit
ATTCTCTCCTCACCTACCTGTGTCGGTTTGCGGTACGGGTACCTCTGCTC178140.1263462768878587No Hit
CGGGAACGTATTCACGGCAGTATGCTGACCTGCCATTACTAGCAATTCCG171860.12189216989978331No Hit
CTCCACTATCGGTCACCAGTTAGTATTTAGCCTTGGAGGGTGGTCCCCCC171090.12134604531685049No Hit
CTTAGGTCCCGACTTACCCTGGGCGGACGAACCTTCCCCAGGAAACCTTA168520.1195232658647241No Hit
GTGTAATGCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAG167730.11896295622768913No Hit
CTTAGGCTTTCGACGGTAAAGATTCTCACTTTACTTTCGCTACTTATTCC165660.1174948031281165No Hit
CCACTTCTCTCAAACGAGCAGCGGTAGTACAGGAATTTCAACCTGTTGTC162220.11505497382254654No Hit
CTAAGCCCTACTTTCGTACCTGCTCGACTTGTCTGTCTCGCAGTCAGGCT156930.1113030270125276No Hit
CAACCAGTTGTCCATCGCCTACGCATTCTCTGCCTCGGCTTAGGCCCCGG155840.11052994156396038No Hit
CTTCTCTCAATCGAGTAACGGTGGTACAGGAATTTCAACCTGTTGCCCAT155050.10996963192692541No Hit
GGAATTTCAACCTGTTGTCCATCACCTACGCCTTCCGGCCTCGGCTTAGG154260.10940932228989043No Hit
GGATTCTCTCCTCACCTACCTGTGTCGGTTTGCGGTACGGGTACCTCTGC152450.1081255748936458No Hit
GGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGG151550.10748724745904899No Hit
CGAAACAACCGGCGGATTTCCCTACCGGTCTATCTACCTCCTTTGGACGA148850.1055722651552586No Hit
GCTGACTGCGTTTTAATACCGGTTGACGGGTGTCAAATTGGAGCGGTAAT147160.10437362808362685No Hit
CTTGAGGCTAGCCCTAAAGCTATTTCGGGGAGAACCAGCTATATCCGAGT145920.10349415472929349No Hit
AGGAATTTCAACCTGTTGTCCATCACCTACGCCTTCCGGCCTCGGCTTAG143800.10199053899446549No Hit
CTACGCCTTCCGGCCTCGGCTTAGGTCCCGACTTACCCTGGGCGGACGAA143110.10150115462794129No Hit
CTCTACTGCCATAGCTTCGGTACACAGTTTAGCCCCGAACATTTTCGGCG141620.10044436809733104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)