FastQCFastQC Report
Wed 3 Jul 2024
SRR6820491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6820491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14099347
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGAATAAGTAGCGAAAGTAAAGTGAGAATCTTTACCGTCGAAAGCCT2009491.4252362183865679No Hit
GCCGGTATAAGTAGCGAGAGGCAGGTGAGAATCCTGTCCGCCGAAAGCCT1864571.322451316362382No Hit
CGGCGAGTTATTGTATGCGGCGAGGTTAAGTACTCATAAGGTACGGAGCC1127240.799498019305433No Hit
CCCGGCCATACCAATTACCGCTCCAATTTGACACCCGTCAACCGGTATTA1032960.7326296742678934No Hit
CCGGCGAGTTATTGTATGCGGCGAGGTTAAGTACTCATAAGGTACGGAGC904390.641441053972216No Hit
CCGGAATAAGTAGCGAAAGTAAAGTGAGAATCTTTACCGTCGAAAGCCTA888940.6304830996783042No Hit
GTGGGTTTGCTAAGACAACTAGGATGTTGGCTTAGAAGCAGCCATTCATT878500.6230785014369814No Hit
CCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCT852410.6045740983607255No Hit
CCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA831120.5894741082689858No Hit
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCC818100.5802396380484856No Hit
CCGGTATAAGTAGCGAGAGGCAGGTGAGAATCCTGTCCGCCGAAAGCCTA794290.5633523311398747No Hit
CCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCC752340.5335991801606131No Hit
CGGAATAAGTAGCGAAAGTAAAGTGAGAATCTTTACCGTCGAAAGCCTAA731400.518747428515661No Hit
CGGGGCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGGG708200.502292765757166No Hit
CGGTATAAGTAGCGAGAGGCAGGTGAGAATCCTGTCCGCCGAAAGCCTAA697950.49502292552981353No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG668650.47424182127016234TruSeq Adapter, Index 1 (97% over 35bp)
GCCGGAATGAGTAGCGAGAGTTAAGTGAGAATCTTGACCGCCGAAAGCCT617580.4380202856203198No Hit
CTGGGAGTCAGACTATGGGGGATAAGCTTCATAGTCGAGAGGGGAACAGC616430.43720464500944617No Hit
CTGGGCTACACACGTACTACAATGGCTGCTACAAAGGGAAGCGATACCGC590740.4189839430152333No Hit
GGCGAGCGAAAGCGGAAGAGCCCAAACCGAGCTATAGCAATATAGTTCGG578810.4105225582433002No Hit
GGTATAAGTAGCGAGAGGCAGGTGAGAATCCTGTCCGCCGAAAGCCTAAG555740.3941600983364691No Hit
GGCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAG510010.3617259721319009No Hit
CGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACC507300.3598038973010594No Hit
GTTTGCTAAGACAACTAGGATGTTGGCTTAGAAGCAGCCATTCATTCAAA458700.32533421583283256No Hit
GTCCGGGGCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTA418570.2968719047768666No Hit
CCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG415640.29479379435090153No Hit
GTCGGTTCTTTCCATCCTGGGTGTGCAGCAGCACCCAAGGGTGGGGTTGT405970.2879353206925115No Hit
GGGGAGCTTACTGTGGTAGGCCGAAGCATGACCGGAAGGACATGTGGACG399490.2833393631634146No Hit
CCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTC386090.27383537691497345No Hit
CCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTT378220.26825355812577706No Hit
GGCGAAGGTTACATGTGAATGTAGCTGGACTTCACAGAAGTGAGAATCCT358090.25397630117196207No Hit
CCGGGGCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGG335850.2382025210103702No Hit
GCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAGC335350.23784789465781642No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGTCCAA333810.23675564549195083No Hit
GTCGCAGCTACAAGTAGCAATGTATTGAAAAATCCACGTACCAATCCATG329870.23396118983382708No Hit
GGAATAAGTAGCGAAAGTAAAGTGAGAATCTTTACCGTCGAAAGCCTAAG313650.22245711095698262No Hit
CTCAAGGTTCACACCGAAGCTGCGGATGCTAAGTGTAGCTTAGCGTGGTA306600.2174568793859744No Hit
GTCCTGGGCTACACACGTACTACAATGGCTGCTACAAAGGGAAGCGATAC301990.21418722441542862No Hit
CTGACGGGGAGGGAAAAATAAGTACCGAAGTGACCGATTCCGCACTGACA298890.2119885410295952No Hit
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA292800.20766919205549023No Hit
CCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGG284990.20212992842860025No Hit
CGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAGCTTACTGTGGTAGGC282840.20060503511261904No Hit
GGCGAGGTTAAGTACTCATAAGGTACGGAGCCGAAGGGAAACCGAGTCTT282470.20034261161172925No Hit
CGATAGTGGAGAAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGG278290.1973779353043797No Hit
CTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAAC272000.1929167357892532No Hit
GTATAAGTAGCGAGAGGCAGGTGAGAATCCTGTCCGCCGAAAGCCTAAGG271850.19281034788348708No Hit
GGGTTTGCTAAGACAACTAGGATGTTGGCTTAGAAGCAGCCATTCATTCA269850.19139184247327198No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT269710.19129254709455693TruSeq Adapter, Index 1 (97% over 36bp)
CGCAGCTACAAGTAGCAATGTATTGAAAAATCCACGTACCAATCCATGCA263000.18653346144328528No Hit
CCCGAACCGAATTGACGTTGAAAAGTCATGGGATGAGCTGTGGGTAGGGG259950.18437024069270724No Hit
GTCCGGAATTACTGGGTGTAAAGGGCGCGTAGGCGGGGATGCAAGTCAGA245590.17418537184736288No Hit
GTCTAGATGTTGCTTGGGAGTCAGACTATGTGAGATAAGTTTCATAGTCG242850.17224201943536818No Hit
CTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCC240160.17033412965862887No Hit
CCGTTATCGTCGCAGCTACAAGTAGCAATGTATTGAAAAATCCACGTACC239820.1700929837388923No Hit
CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCG234660.16643323978053737No Hit
CCGGCCATACCAATTACCGCTCCAATTTGACACCCGTCAACCGGTATTAA231740.1643622218816233No Hit
CCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAGCTTACTGTGGTAGG230140.16322741755345122No Hit
CCGATAGTGGAGAAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGG229070.16246851715898616No Hit
CGGGGCTCAAGGTTCACACCGAAGCTGCGGATGCTAAGTGTAGCTTAGCG219690.15581572678507735No Hit
CCGGCATAATGATTCTACGAGCTTAGCAGAAGTAAGCTGGAAAGCTTACA219140.1554256377972682No Hit
GTGCCGAATTGCGGATAGGCGAGCGAAGGTATAAGGTAGCAGAACACTGC218580.15502845628240797No Hit
GGCGAGCGAAAGCGGAGGAGGCCAAACCATATTACTTCGGTAATATGGGG216150.15330497220899664No Hit
CCGGGATCCAGAGTACCACGAGGCACGTGGAACCTTGTGGGAAGCAGGGG210080.1489998082889938No Hit
ACCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAGCTTACTGTGGTAG204060.14473010700424635No Hit
GGCGAGGTTAAGTACTTAAGGTACGGAGCCGCAGGGAAACCGAGTCTGAA203420.14427618527297753No Hit
CGGCGAGTTACATTGTAAGGCGAGGTTAAGTACTTAAGGTACGGAGCCGC202530.14364495036543182No Hit
GCCGTCTAGATGTTGCTTGGGAGTCAGACTATGTGAGATAAGTTTCATAG199380.14141080434434303No Hit
CGCCGAAGATGTAACGGGGCTAAGCGTAGCACCGAAGCCATAGACTTACT198330.14066608900398012No Hit
GGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCCCAA194000.13759502479086444No Hit
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCA192610.13660916353076494No Hit
GGGCTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGA187980.133325323506117No Hit
CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGC185080.13126849066130508No Hit
CCTGGGAGTCAGACTATGGGGGATAAGCTTCATAGTCGAGAGGGGAACAG178380.12651649753708452No Hit
CTAAACTGTGTACCGAAGCTATGGCAGTAGAGATACTGGGTAGGGGAGCT175910.12476464335546887No Hit
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG175470.12445257216522154No Hit
CGACTGTTTAATAAAAACATAGCTCCTAGCTAGCCCGTGAGGGTGTGTAC174070.12345961837807097No Hit
GTCCGGAATTACTGGGTGTAAAGGGCGTGTAGGCGGGGGTGCAAGTTACT170470.12090630863968381No Hit
GGGGACGCAGGGGGATAGGTCAAGCGAGCGACCGGAAGAGCTCGTCCAAA168530.11953035839177516No Hit
GGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCC168430.1194594331212644No Hit
GGGGAACCCACCTGAGGTAAATGCTCAGGTATCCGTACATGAACACATAG168100.11922537972857891No Hit
GTGGTAAGCTGCGATAAGCTGCGGTGAGGCGCAAACAGCCATTGACCCGC167410.11873599536205472No Hit
CGGCGAGGTTAAGTACTCATAAGGTACGGAGCCGAAGGGAAACCGAGTCT165110.11710471414030735No Hit
GGGGAGGGAAAAATAAGTACCGAAGTGACCGATTCCGCACTGACAAGAAA164040.11634581374584227No Hit
GGGTAGGGGAGCTTACTGTGGTAGGCCGAAGCATGACCGGAAGGACATGT163850.11621105573187183No Hit
GGAGCTTACTGTGGTAGGCCGAAGCATGACCGGAAGGACATGTGGACGAG163780.11616140804251432No Hit
CGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCT162540.11528193468818096No Hit
CGAAGATGTAACGGGGCTAAGCGTAGCACCGAAGCCATAGACTTACTTTA160680.1139627246566809No Hit
GTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT157860.11196263202827764No Hit
CTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTC154160.1093383970193797No Hit
GGTCCGGCGAGTTATTGTATGCGGCGAGGTTAAGTACTCATAAGGTACGG153770.10906178846438776No Hit
CGGGGAGGGAAAAATAAGTACCGAAGTGACCGATTCCGCACTGACAAGAA153460.1088419201258044No Hit
CCGGCGAGTTACATTGTAAGGCGAGGTTAAGTACTTAAGGTACGGAGCCG152770.10835253575928021No Hit
CGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG150000.1063879057661323No Hit
GTCCGGCGAGTTATTGTATGCGGCGAGGTTAAGTACTCATAAGGTACGGA149170.10579922602089302No Hit
GGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGAG148500.10532402670847096No Hit
CCCGGGTACCGAGCTCGAATTCGCCCTATAGTGAGTCGTATTACAATTCA147490.10460768147631234Clontech Universal Primer Mix Long (95% over 23bp)
CACTGATTATGTCGATTGACCTGAAGGGGAGACGCAGGAAGGTAGCTACG146090.10361472768916177No Hit
CCGGAATTACTGGGTGTAAAGGGCGCGTAGGCGGGGATGCAAGTCAGATG145170.10296221520046282No Hit
CCGGAATGAGTAGCGAGAGTTAAGTGAGAATCTTGACCGCCGAAAGCCTA144250.10230970271176389No Hit
GACCGATAGTGGAGAAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGA143480.10176357812883108No Hit
GTCCCGTATGCGAAATGCGAAGTGACAGGCCAGGATCCAGAGTACCACGA142890.10134511903281762No Hit
CTTGGGAGTCAGACTATGTGAGATAAGTTTCATAGTCGAGAGGGAAACAG142480.10105432542372353No Hit
CCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTG142450.10103304784257029No Hit
CCCGACTGTTTAATAAAAACATAGCTCCTAGCTAGCCCGTGAGGGTGTGT141670.10047983073258641No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)